uHPLC Simulation        
   
1st Column   Given Parameters   2nd Column   Calculated Parameters
   
Length ID     Length ID  
cm mm   cm mm  
   
           
Flow rate   Linear Flow t0   Flow rate   Increased Flow t0
mL/min µL/min cm/min min   ml/min µL/min x Factor min
 
   
Particle Size Pressure       Particle Size Pressure    
µm bar Mpa Psi   µm bar Mpa Psi
 
   
Peak 1   Peak 2     Peak 1   Peak 2  
Ret.Time [min] Ret.Time [min]   Ret.Time [min] Ret.Time [min]
K-Value K-Value   K-Value K-Value
Plates/Column Plates/Column   Plates/Column Plates/Column
Volume [µL] Volume [µL]   Volume [µL] Volume [µL]
     
Injection Volume Loading   Concentration   Injection Volume Loading   Concentration
µL µg ng µg/mL   µL µg ng µg/mL
 
     
Flow cell   Selectivity α   Flow cell   Selectivity α
Path length [mm] Resolution   Path length [mm] Resolution
Volume [µL] Sensitivity 1,00   Volume [µL] Sensitivity
      Sensivity Resolution Pressure Time
 
Gradient    
1st Linear Down-Scaling      
With same flow rate the gradient in the relationship of the column lengths is shortened and/or extended  
Gradient Column 1     Gradient Column 2   Pressure  
Flow Rate (mL/min)   Flow Rate (mL/min) bar psi
Time (min) % B   Time (min) % B
   
   
   
   
   
   
   
   
   
2nd Constant Gradient Volume    
Independently of the column length the same eluent volume is always rinsed through the column  
Gradient Column 1     Gradient Column 2   Pressure  
Flow Rate (mL/min)   Flow Rate (mL/min) bar psi
Zeit (min) % B   Zeit (min) % B
   
   
   
   
   
   
   
   
   
   
Vers.:4.6 © Jürgen Maier-Rosenkranz   info@jmr-analytik.de

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